Fig. 5
From: Abnormal arginine synthesis confers worse prognosis in patients with middle third gastric cancer
ASS1 was up-regulated in gastric cancer, and knockdown of ASS1 inhibit progression of gastric cancer. (A) TCGA data analysis showed that ASS1 was significantly up-regulated in gastric cancer tissues, and also showed an upward trend in middle-third gastric cancer compared to the tumors located in other locations. C, gastric cancer tissues; N, normal tissues. (B) Expression level of ASS1 in various gastric cancer cells. (C) Western blot successfully confirmed the knockdown of ASS1 in gastric cancer cell lines HGC-27 and MKN28. (D) CCK8 assay showed that knockdown of ASS1 significantly suppressed the proliferation of gastric cancer cells. (E) Colony formation assay showed that knockdown of ASS1 significantly inhibit the clone forming ability of both gastric cancer cells. (F) Transwell assay showed that knockdown of ASS1 significantly inhibit the migratory ability of gastric cancer cells. (G) Knockdown of ASS1 significantly promoted the apoptosis of gastric cancer cells. All the data were shown as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001