Fig. 2

RORα deletion promotes GC proliferation and is reversed by SR1078. A RORα expression levels were detected by western blot in lentiviral transfected AGS, MKN-74 and MFC cells. β-actin as a loading control. B The colonies of GC cells were performed with colony formation assay. N = 3. C The absorbance value of RORα-KO GC cells or GC cells treated with SR1078(5 µM) according to time gradient through CCK-8 assay. N = 3. D The relative mRNA levels of E-cadherin, N-cadherin and Vimentin were detected by qPCR assay in RORα-KO GC cells or GC cells treated with SR1078(5 µM). N=3. E The expression levels of Ki-67 and PCNA were detected through immunohistochemistry stain and MOD method in subcutaneous tumor. Original 200 and 400 magnification. Scale bar = 100 μm. N = 3. The RORα-KO-MFC cells were injected into subcutaneous flank of mice until the tumor volume reached 100 to 300 mm³. F The expression levels of Ki-67 and PCNA were detected through immunohistochemistry stain and MOD method in subcutaneous tumor. Original 200 and 400 magnification. Scale bar = 100 μm. N = 3. The MFC cells treated with SR1078 (5 µM) were injected into subcutaneous flank of mice until the tumor volume reached 100 to 300 mm³. All data are represented as the mean ± standard deviation. Vehicle was PBS. *P < 0.05, **P < 0.01, and ***P < 0.001