Fig. 7

High proliferation and high glucose inhibit RORα expression in GC cells. A The colonies of GC cells treated with TGF-β1 (10ng/ml) were performed with colony formation assay. N = 3. B The absorbance value of GC cells treated with TGF-β1 (10 ng/ml) according to time gradient through CCK-8 assay. N = 3. C The relative mRNA levels of E-cadherin, N-cadherin and Vimentin were detected by qPCR assay in GC cells treated with TGF-β1 (10 ng/ml). N = 3. D RORα protein expression levels were detected by western blot in GC cells treated with TGF-β1 (10 ng/ml). E RORα protein expression levels were detected by western blot in GC cells treated with glucose in concentration gradient. All data are represented as the mean ± standard deviation. Vehicle was DMSO. β-actin as a loading control. *P < 0.05, **P < 0.01, and ***P < 0.001