Fig. 6From: An estrogen-regulated long non-coding RNA NCALD promotes luminal breast cancer proliferation by activating GRHL2LncRNA NCALD may interact directly with ERα. Cell immunofluorescence assay revealed the colocalization of lncRNA NCALD with ERα in MCF7 and T47D cells (panel a). RNA pull-down assay (b, c) and RIP (d) assay indicated a significant interaction between ERα and ncRNA NCALD (n = 3, ***P < 0.001) (panels b–d). The results of the RNA pull-down (e) and RIP (f) assays demonstrated that the fragment spanning nucleotides 1-121 of ERα interacted with lncRNA NCALD at position 1-151 (n = 3, **P < 0.01) (panels e–f)Back to article page