Fig. 4
From: Peroxiredoxin 3 regulates breast cancer progression via ERK-mediated MMP-1 expression
MMP1 plays an important role in PRDX3-mediated breast cancer tumorigenesis. (A) Real-time RT-PCR of the mRNA levels of MMP1 in sh-NT, sh-PRDX3C, EV and PRDX3-OE stable transfected cells. (B) Enzyme-linked immunosorbent assay (ELISA) of MMP1-secrected activity was evaluated in conditioned medium (CM). (C) Western blot analysis of MMP1 protein level in conditioned medium (CM) and whole cell lysates (WCL). GAPDH was used as loading control for WCL. Each bar represents mean ± SEM, n = 3. **P < 0.01, ***P < 0.001