Fig. 5

Phf6 deficiency decreases the activity of leukemia stem cells in AML (A-B) GFP analysis in BM (A) and spleen (B). n = 4, Student’s t-test; (C) Analysis of c-Kit⁺ spleen cells. n = 4, Student’s t-test; (D) Analyses of L-GMP in spleen cells. n = 3, Student’s t-test; (E-F) The percentage (E) and absolute number (F) of c-Kit⁺ AML cells in BM. n = 4, Student’s t-test; (G-H) The percentage (G) and absolute number (H) of L-GMP in BM. n = 3, Student’s t-test; (I-J) Colony-forming of AML cells from P1 (I) and P2 generation (J). Type A colonies are very compact without a halo of migrating cells. Type B colonies have a compact center and a halo of single cells. Type C colonies have no center and only single cells. n = 3, Student’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001