Fig. 7
The Knockdown of ANP32B Resulted in a Significant Decrease in the Levels of Acetylated Histone H3 in AML Cell Lines. A, B Western blotting analysis showed that ANP32B knockdown caused a substantial decrease in global acetyl-H3 and acetylation of histone H3k27. C Heat map generated by CUT&Tag data analysis. CUT&Tag technology showed that a decrease in the overall occupancy of H3K27ac across the entire genome following ANP32B knockdown. D IGV visual analysis showed a reduction in H3K27ac signaling in genes involved in the MYC signaling pathway. E Box plots for log2 fold-change in mRNA expression of genes and H3K27ac enrichment signals with all genes enrichment (grey) or MYC pathway enrichment (blue). F The volcano plot analysis showed that the MYC pathway co-existed in log2 fold-change in mRNA expression and H3K27ac enrichment signals. G Schematic representation of ANP32B in the pathogenesis of AML