Fig. 4

PPP2R1B regulates CRC cells metastasis by MAPK/ERK signaling pathway. A Protein of PPP2R1B, ZEB1, E-cadherin, Snail, p-ERK and ERK change in SW480 in PPP2R1B siRNA group and negative control group with or without PD98059. B Quantitative analysis of the Western Blot was performed for SW480 cells using ImageJ software. C Representative images of SW480 cells transfected with control, PPP2R1B knockdown and PPP2R1B knockdown plus PD98059 in migration and invasion assays at ×20 magnification. D Quantitative analysis of the numbers of cells was performed for SW480 cells using ImageJ software. E A wound healing assay was performed in SW480 cells with control, PPP2R1B knockdown and PPP2R1B knockdown plus PD98059. Representative images at the indicated time points are shown at magnification ×10. F Quantitative analysis of the migration area was performed for SW480 cells using ImageJ software. Bars indicate Mean ± S.E. *P < 0.05, **P < 0.01 and ***P < 0.001 compared with the control, n = 3