Fig. 5

LASS2 overexpression alters the ferroptosis status of different cell lines through dysregulation of iron homeostasis. A KEGG categorization plot of differentially abundant metabolites. The y-axis shows the name of the KEGG metabolic pathway, and the x-axis shows the number of differentially abundant metabolites under the altered pathway, with their number as a proportion of the total number of metabolites annotated. B Metabolite set enrichment analysis (MSEA) of the metabolic datasets. C KEGG enrichment scatterplot of differentially expressed genes. D–G Changes in the expression of key proteins involved in ferroptosis and iron homeostasis in different tumour cell lines, as determined by western blotting. H–K The intracellular Fe²⁺ concentration was measured via fluorescence imaging and flow cytometry (scale bar: 100/50 μm). *P < 0.05, **P < 0.01, ***P < 0.001