Fig. 4

SEC63 promoted tumor growth and metastasis in gastric cancer. (A) The mRNA level of SEC63 in gastric cancer and corresponding paracancerous tissues was measured by qPCR assay. (B) Western blotting assay was used to detect the SEC63 protein level in gastric cancer and corresponding paracancerous tissues. (C-D). SEC63 overexpression plasmid transfection efficiency was verified by qPCR assay and western blotting assay respectively. (E). CCK-8 assay was used to measure the proliferation ability change induced by SEC63 overexpression. (F). Colony formation assay was used to measure the tumorigenesis ability affected by SEC63 overexpression. (G-H). Migration and invasion change induced by SEC63 overexpression were detectd by wound healing and Transwell assay at the context of WFDC21P inhibition. (I) The key protein levels in EMT were measured by western blotting after WFDC21P downregulation and SEC63 overexpression. The data are presented as the mean ± SD of at least three independent experiments. ns, not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001