Fig. 3

Comparison of the cellular vitality and cytotoxicity of eHD-NKs and eAML-NKs upon tumor cell lines. A, B FCM diagrams (A) and statistical analysis (B) of the apoptotic cells (7-AAD⁻Annexin V⁺, 7-AAD⁺Annexin V⁺, Annexin V⁺) in eHD-NKs and eAML-NKs. C, D FCM diagrams (C) and statistical analyses (D) of the distribution of the indicated sub-stages (G0/G1, S, G2/M) in cell cycle. E, F FCM diagrams (E) and statistical analysis (F) of CD107a⁺ NK cells in the Ctr group (monoculture) and the experimental groups cocultured with diverse tumor cell lines (K562, Nalm6, U937) at the effector-to-target ratio (E: T = 1:3). G, H Comparison of the ex vivo cytotoxicity of eHD-NKs and eAML-NKs against the indicated tumor cell lines (K562, Nalm6, U937) (E: T = 1:3). All data were shown as mean ± SEM (N = 3 independent experiments). All data were shown as mean ± SEM (N = 3 independent experiments). *P < 0.05; **P < 0.01; NS: not significant