Fig. 3

C6orf15 promotes invasion and proliferation and induces the epithelial–mesenchymal transition (EMT) in CRC. a Enrichment analysis of signalling pathways based on GSEA-Hallmark database. b Western blots show the expression of C6orf15 in different cell lines. c qRT-PCR was used to verify the gene expression efficiency after the transfection of lentiviruses to silence or overexpress C6orf15. d and e Western blots show the expression changes of EMT-related markers after upregulating and downregulating C6orf15 expression. f Transwell assay results show that C6orf15 overexpression enhanced CRC migration ability and C6orf15 downregulation reduced CRC migration potential. g MTT assays were used to determine the proliferation ability of RKO cells and HCT116 cells transfected with lentiviruses to silence or overexpress C6orf15. For all statistical results, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, and ns (no statistical significance)