Fig. 4

C6orf15 activates the WNT/β-catenin signalling pathway, promoting the nuclear translocation of β-catenin. a Signalling pathway enrichment analysis based on GSEA-Hallmark data showed that C6orf15 was positively correlated with the WNT signalling pathway. b and d Western blot results indicate that the upregulation of C6orf15 expression promotes β-catenin nuclear translocation. c TOPflash-Luciferase reporter gene assay results show that TCF/LEF1 transcriptional activity significantly increased after C6orf15 overexpression. For all statistical results, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, and ns (no statistical significance)